Selectivity and Detectability Optimizations in Hplc

The two primary goals of chromatographers are selectivity and detectability optimizations. However, no text currently exists that deals completely with this subject area. Chromatographic theory does not offer enough support either. Separation on an HPLC column is a complex process. The functional groups of chemical compounds, isomeric structures, or high molecular weight can also influence separations. As part of the continuing series in Chemical Analysis, Selectivity and Detectability Optimizations in HPLC provides a basic understanding of these processes and builds on the data available from conventional approaches to optimization. One major aspect covered is the new opportunities offered by computers to achieve the desired optimizations. The scope of selectivity and detectability optimizations is described in Chapter 1. The next two chapters treat the physicochemical basis of retention and the studies performed to improve our understanding of separation mechanisms. Chapter 4 provides background information by discussing the conventional approaches to mobile-phase selection and optimization. Chapters 5-8 examine approaches used to optimize separation by adsorption/normal-phase, reversedphase, ion-exchange, and ion-impairing chromatography. Applications in select areas such as macromolecules-especially in the field of biotechnology-and isomers (including chiral separations) are covered in Chapters 9 and 10. Computerized approaches to selectivity in Chapter 11 explore unattended optimizations that could further improve our knowledge in this area. Chapters 12 and 13 treat selective detectors and approaches to optimize detectability. Selectivity and Detectability Optimizations in HPLC will help analytical chemists working in the pharmaceutical, chemical, petroleum, and polymer fields, as well as FDA, USDA, EPA, and other governmental agencies involved in HPLC testing to reduce method development time. In addition, advanced undergraduate and graduate students will gain a better understanding of HPLC separation and detection.Ahuja, Satinder is the author of 'Selectivity and Detectability Optimizations in Hplc' with ISBN 9780471626459 and ISBN 0471626457.