1196092

9781588291240

Membrane Protein Protocols Expression, Purification, and Characterization

Membrane Protein Protocols Expression, Purification, and Characterization
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  • ISBN-13: 9781588291240
  • ISBN: 1588291243
  • Publisher: Humana Press

AUTHOR

Selinsky, Barry S.

SUMMARY

A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.Selinsky, Barry S. is the author of 'Membrane Protein Protocols Expression, Purification, and Characterization' with ISBN 9781588291240 and ISBN 1588291243.

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