Lab Ref A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench

Introduction SECTION 1: QUANTITATIVE NUCLEIC ACID ANALYSISIsolation of Total DNA Isolation of Total RNA Selection of Poly(A) RNA from Total Isolation and Amplification of RNA from Single Cells and Small Amounts of Tissue Hybridization Analysis Preparation of Nucleic Acid (Target) for Array Hybridization Analysis Preparation of Nucleic Acid (Probe) for Array Hybridization Analysis Other Quantitative Methods SECTION 2: RNAiRNAi in C. elegansRNAi in D. melanogasterRNAi in Plants RNAi in Mammalians RNAi in Mouse Oocytes and Early Embryos SECTION 3: IMAGINGPreparation of Embryos for Live Cell Imaging Imaging Fixed Tissue Preparation of Mice for Imaging Intracellular Imaging General Information for Imaging SECTION 4: PROTEIN ANALYSIS/PROTEOMICSBasic Methods in Protein Analysis for Proteomics Extraction of Proteins Advanced Protocols in Identifying Protein–Protein Interactions for Proteomics SECTION 5: ARABIDOPSISMicroscopy Genetic Manipulation Protein Extraction Nuclear Extraction Histochemistry of GUS Fusion SECTION 6: MURINEIn Vitro Culturing of Embryonic Stem Cells Genotyping Immunohistochemistry of Whole-mount Embryos Other Buffers and Solutions SECTION 7: STORAGE AND SHIPMENT OF BIOLOGICAL SAMPLESSECTION 8: USEFUL FACTS AND TABLESA. Useful Information and Reference Tables B. Nomenclature Guide C. Useful World Wide Web Sites AppendixIndexMellick, Albert S. is the author of 'Lab Ref A Handbook of Recipes, Reagents, and Other Reference Tools for Use at the Bench', published 2006 under ISBN 9780879698157 and ISBN 0879698152.