Gene Targeting and Embryonic Stem Cells

Embryonic Stem (ES) cells are increasingly proving their value, not only in research aimed at understanding gene function and human disease, but also as a potential means to treat these diseases. Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Details are provided on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, Human embryonal carinoma cells and Human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.McWhir, Jim is the author of 'Gene Targeting and Embryonic Stem Cells', published 2004 under ISBN 9781859963609 and ISBN 1859963609.